Reporter

Part:BBa_K1431834:Experience

Designed by: Yicong Tao, Yushan Zhang   Group: iGEM14_SUSTC-Shenzhen   (2014-10-14)

Applications of BBa_K1431834

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431834 and BBa_K1431824 whose RBS is BBa_B0031, a relatively weak RBS.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

SUSTC_31_916-1.jpg


At 1:50 (16th,Oct.)

SUSTC_31_916-2.jpg


At 3:00 (16th,Oct.)

SUSTC_31_916-3.jpg


At 6:00 (16th,Oct.)

SUSTC_31_916-4.jpg


At 7:40 (16th,Oct.)

SUSTC_31_916-5.jpg


At 8:00 (16th,Oct.)

SUSTC_31_916-6.jpg


At 10:00 (16th,Oct.)

SUSTC_31_916-7.jpg


At 11:20 (16th,Oct.)

SUSTC_31_916-8.jpg

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC_31_916_L-1.jpg


At 02:45 (17th,Oct.)

SUSTC_31_916_L-2.jpg


At 04:55 (17th,Oct.)

SUSTC_31_916_L-3.jpg


At 10:40 (17th,Oct.)

SUSTC_31_916_L-4.jpg

Conclusion

With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.


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