Composite
LacI-T

Part:BBa_K143062:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-01)

LacI repressor protein - Terminator



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.

Source

LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator.