Generator
Ph-s-gsiB

Part:BBa_K143054:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-08)

Promoter hyper-spank and RBS gsiB for B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-gsiB were obtained from papers1 and the sequence synthesised by GeneArt

Source

Phyperspank-gsiB was synthesised by GeneArt

References

<biblio>

  1. 1 pmid=9669336

</biblio>