RNA

Part:BBa_K1427001:Design

Designed by: Connor McBrine   Group: iGEM14_Tufts   (2014-10-17)


merRNA, c-di-GMP aptamer with promoter and terminators


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 474
    Illegal NheI site found at 502
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 promoter initiates transcription 2 nucleotides (GG) upstream of the actual merRNA sequence. Predictive RNA folding programs did not report any change in secondary structure with this small 5' addition.


Source

Bdellovibrio bacteriovorus

References