Protein_Domain

Part:BBa_K1422502:Design

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-13)


Zea mays Nicotianamine aminotransferase (corn NAAT)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1309
    Illegal SpeI site found at 814
    Illegal PstI site found at 894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal NheI site found at 1124
    Illegal SpeI site found at 814
    Illegal PstI site found at 894
    Illegal NotI site found at 224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1309
    Illegal SpeI site found at 814
    Illegal PstI site found at 894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1309
    Illegal SpeI site found at 814
    Illegal PstI site found at 894
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Bacillus subtilis by altering the 23rd, 109th, and 168th amino acid sequences from CTA to CTT.


Source

The original sequence was obtained from the NCBI website. NCBI. (19 October, 2011). Zea mays ZmNAAT1 mRNA for putative nicotianamine aminotransferase, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/166788521

References