RBS

Part:BBa_K1419003:Design

Designed by: Harland Brandon   Group: iGEM14_Lethbridge   (2014-10-09)

Universal riboregulatable RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needed to alter the natural RNA-OUT design to allow for base pairing to the RBS.


Source

Modifications were made to the B0034 part using 'Round-the-horn mutagenesis.

References