Generator

Part:BBa_K1418061:Design

Designed by: Ryan Putman   Group: iGEM14_Utah_State   (2014-10-16)


phaCAB-p-GFPPhaC-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 916
    Illegal BglII site found at 1741
    Illegal BglII site found at 5761
    Illegal BamHI site found at 4965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal NgoMIV site found at 293
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1205
    Illegal NgoMIV site found at 1484
    Illegal NgoMIV site found at 2136
    Illegal NgoMIV site found at 2158
    Illegal AgeI site found at 5460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4002


Design Notes

GFPmut had stop codon removed to generate fusion protein


Source

Composite

References