Part:BBa_K1412010:Experience
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Applications of BBa_K1412010
chemotaxis towards hyperosmotic pressure, a new killing device.
User Reviews
UNIQ375969f9bfc7869e-partinfo-00000000-QINU
XMU-China iGEM 2014 |
Figure 1. The plot of Moving radius versus Sucrose concentration. The four curves were measured after 10h, 11h, 12h and 16.5h respectively. Figure 2. Drawing horizontal line with 10% sucrose and erect line with water. Spotting cells on the cross. Two plates are parallel experiment. Culturing for 48 hours, we observed that reprogrammed E.coli has significant orientation to high concentration line. As high concentration sucrose generates high hyperosmosis, it has proved that CL-1 has the tendency swimming to high osmotic pressure.
We use semi-solid medium culture with gradient concentration of sucrose to characterize the device (BBa_K1412010). Setting the motile ability is proportional to the moving radius. From the plot, when no sucrose added in, the motile ability is the weakest. The motile ability keeps growing while the concentration of sucrose increased from 0 to 4%. Then the motile ability went down slightly as the sucrose concentration increased from 4% to 10%, but is still stronger than that at concentration 0. We can make the conclusion that our device is working as expectation, the motile ability went down because of the inhibition from hyperosmotic pressure. Besides, for even at the inhibiting osmotic pressure, the motile ability is still stronger than that without any inducer, reprogrammed CL-1 may even swim to killing osmotic pressure which will kill bacteria itself. |
UNIQ375969f9bfc7869e-partinfo-00000001-QINU
More information, click here: [http://2014.igem.org/Team:XMU-China/Project_Application_BlackHole# XMU-China Black Hole]
References
[1][http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05048.x/abstract;jsessionid=DB389E887A1738CF004BAD1B61A6D336.f01t01 Batchelor, Eric, and Mark Goulian. "Imaging OmpR localization in Escherichia coli." Molecular Microbiology 59.6(2006):1767–1778.]
[2][http://www.ncbi.nlm.nih.gov/pmc/articles/PMC216723/ Kawaji, H., T. Mizuno, and S. Mizushima. "Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12." Journal of bacteriology 140.3 (1979): 843-847.]