Coding

Part:BBa_K1409000:Design

Designed by: Andy Halleran   Group: iGEM14_William_and_Mary   (2014-10-08)


ChR1 from volvox


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 901
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed one PstI cutsite from the coding region for BioBrick compatibility.


Source

Deissroth lab.

References

Zhang, Feng, et al. "Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri." Nature neuroscience 11.6 (2008): 631-633.