Coding

Part:BBa_K1408001:Design

Designed by: Stephen A Rettie   Group: iGEM14_Washington   (2014-10-09)


Gal4-Vp16 Transcriptional Activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 338
    Illegal XhoI site found at 83
    Illegal XhoI site found at 566
    Illegal XhoI site found at 1105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 485


Design Notes

Xba1, EcoR1 and Spe1 restriction sites were present in the original sequence obtained from the Fields Lab. The submitted part was synthesized with synonymous substitutions (silent mutations) amplified with primers containing the biobrick prefix and suffix and then digested and ligated into the iGEM vector.

This is an improved part over https://parts.igem.org/Part:BBa_K1179014 which was found to have an illegal restriction site in the VP16 part of its sequence.

Source

Gal4 is a DNA binding fragment from Saccharomyces cerevisiae. VP16 is a transcriptional activator from herpes simplex virus. This part was obtained from the Fields Lab at the University of Washington,

References

Degron from Ben Jester - Fields Lab, UW