Part:BBa_K1405008:Design
A Kill Switch with "memory" time repressed by IPTG
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is better to divide it into three parts according to the main page information. And establish each parts in different plasmid,since it will be easier to control the expression level of proteins.
Source
All parts are from iGEM parts.
References
Hazan R, Engelberg-Kulka H. mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria. Journal of Cell Science 118:4327-4332
Hazan R, Sat B, Engelberg-Kulka H. Escherichia coli mazEF-Mediated Cell Death Is Triggered by Various Stressful Conditions. Journal of Bacterology 186(11):3663-3669.
Schuster CF, Park JH, Prax M.Characterization of a mazEF Toxin-Antitoxin Homologue from Staphylococcus equorum. Journal of Bacteriology 195:115-125
Lewis D, Le P, Zurla C, Finzi L, Adhya S.Multilevel autoregulation of λ repressor protein CI by DNA looping in vitro. PNAS 108:14807-14812.
Wedler H, Wambutt R. A temperature-sensitive lambda cI repressor functions on a modified operator in yeast cells by masking the TATA element. NCBI 248:499-505.