Coding

Part:BBa_K1405005:Design

Designed by: Fengjing，Ningze Sun,Xuan Wang   Group: iGEM14_BNU-China   (2014-10-12)

INPN+ModA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


Design Notes

Using INP derivatives containing only N-domain (INPN) as display system successfully is possible. There is also literature which alleges that since full-length INP is quite large (1,200–1,500 amino acid residues), functional truncated INP molecules may serve as better anchoring motifs to carry large heterologous proteins. InaZ uesd before by other teams has less literature support. So, we use inaK and inaQ as ingredient.


Source

The sequence of inaK can be found in the The National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information(NCBI) and the gene was synthesized by a company.

References