Generator

Part:BBa_K1403015:Design

Designed by: Clovis & François   Group: iGEM14_Paris_Bettencourt   (2014-10-06)


Trimethylamine moonoxygenase (tmm) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 262
    Illegal AgeI site found at 485
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

IDT codon optimised and synthesised as gBlock.


Source

Gene synthesis, codon optimised.

References