Measurement

Part:BBa_K1403001:Design

Designed by: Urszula Czerwinska   Group: iGEM14_Paris_Bettencourt   (2014-10-03)

GFP generator in pSB1C3 under a weak promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

The designed was made according to interlab study requirements. The promoter and the vector have been taken from BBa_BBa_K823012, cut with XbeI and SpeI. The GFP coding sequences as well as RBS and terminators have been cut out from BBa_E0240 also with XbeI and SpeI and introduced to the cut promoter + backbone. We used heatshock competent NEB cels. For more details please check: [http://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study# Interlab study]

Source


kit 2014
BBa_K823012: Plate 1, Well 20K
BBa_E0240: Plate 1, Well 22I

References

http://2014.igem.org/Tracks/Measurement/Interlab_study