Translational_Unit

Part:BBa_K1402002

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-09-29)

T7-RBS-MsbA-Terminator

ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. This ABC transporters MsbA can transport fatty acids out of the cell.The RBS and T7 promoter are in front of the MsbA coding sequence and T7 terminator is after MsbA. You can only use this part in E.coli BL21(DE3) or choose other parts we offer in other strains.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 61
    Illegal XhoI site found at 1521
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1451


[edit]
Categories
Parameters
None