Regulatory

Part:BBa_K1401008:Design

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-10-09)


Tandem Promoter pBad-pTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 60


Design Notes

We looked through the literature and decided to attach the two promoters without any extra sequence between them



Source

This was formed using BBa_I13453 and BBa_R0040


References