Regulatory

Part:BBa_K1400005:Design

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)


pTRE(2) Single input tetr responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mig1 sites were replaced with Tet sites.


Source

This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.

References