Regulatory

Part:BBa_K1400001:Design

Designed by: Dylan Siriwardena   Group: iGEM14_uOttawa   (2014-10-06)


PTRE(2)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The major design consideration was the proximity of the repressing sites to the TATA box. 10bp seemed to cause strong repression while still not interfering too much with transcription when no proteins are bound.


Source

This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.

References