Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Reporter

Part:BBa_K1399016:Experience

Designed by: Anna Stikane   Group: iGEM14_Edinburgh   (2014-09-19)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1399016

We characterized and compared the instability of this SsrA-LAA tagged GFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)

SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).
Summary of BioBricks to which our characterization data is applicable.

User Reviews

UNIQd5a8e41e5096d422-partinfo-00000000-QINU UNIQd5a8e41e5096d422-partinfo-00000001-QINU