Coding

Part:BBa_K1398002:Design

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-11)

NemA (N-ethylmaleimide reductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal AgeI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence of this part was found using the NCBI database and taken from E. coli. The sequence was then codon-optimised to ensure increased expression. This has resulted in a novel synthetic sequence.


Source

Source of original sequence: Escherichia coli O104:H4 str. C227-11

References

Caspi, R., Foerster, H., Fulcher, C., Kaipa, P., Krummenacker, M., Latendresse, M., Paley, S., Rhee, S., Shearer, A., Tissier, C. and others, (2008). The MetaCyc Database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases. Nucleic acids research, 36(suppl 1), pp.623--631.