Coding

Part:BBa_K1393000:Design

Designed by: Jiajun Tan   Group: iGEM14_HUST-China   (2014-10-02)

OprF(Val188)+GS linker+CBP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid. In order to reduce the effect on the CBP activity,we added GS linker between OprF and CBP.

Source

The OprF gene is from Pseudomonas aeruginosa.

References

[1] Seung Hwan Lee,Jong-il Choi,Mee-Jung Han,Jong Hyun Choi,Sang Yup Lee. Display of Lipase on the Cell Surface of Escherichia coli Using OprF as an Anchor and Its Application to Enantioselective Resolution in Organic Solvent. Biotechnology and bioengineering,90(2):223-230.

[2] Rebecca S.Y. Wonga, Robert A. Wirtz b and Robert E.W. Hancock a . Pseudomonas aeruginosa outer membrane protein OprF as an expression vector for foreign epitopes: the effects of positioning and length on the antigenicity of the epitope. Gene, 158 (1995) :55-60.

[3] Sambandam Ravikumar , Ik-keun Yoo , Sang Yup Lee ,Soon Ho Hong. Construction of Copper Removing Bacteria Through the Integration of Two-Component System and Cell Surface Display. Appl Biochem Biotechnol (2011) 165:1674–1681.

[4] Eileen G. Rawling,Nancy L. Martin,Robert e. W. Hancock*.Epitope Mapping of the Pseudomonas aeruginosa Major Outer Membrane Porin Protein OprF. Infect and Immun.1995, 63(1):38-42.