Composite
AraC RBS

Part:BBa_K1392935:Design

Designed by: Şeniz Yüksel   Group: iGEM14_METU_Turkey   (2014-09-29)


AraC-Pbad - Arabinose inducible regulatory promoter/repressor unit+ RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We could easily use this part by binding it with a RBS, to produce an inducible regulatory promoter/repressor unit.


Source

In Escherichia coli, the AraC protein represses transcription from its own promoter, PC, and when it is associated with arabinose, it activates transcription from three other promoters, PBAD, PE, and PFGH. Expression from all four of these promoters is also regulated by cyclic AMP-catabolite activator protein; however, the arrangement of the protein binding sites is not identical for each promoter.

References