Coding
circircido

Part:BBa_K1392931:Design

Designed by: Burak Kızıl   Group: iGEM14_METU_Turkey   (2014-09-29)


2-oxopent-4-enoate hydratase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to take 2-oxopent-4-enoate hydratase from Escherichia coli BL21(DE3)'s complete genome a primer has designed and to get 810 bp, the part has connected to CHL backbone.


Source

Escherichia coli BL21 (DE3)

References

http://www.ncbi.nlm.nih.gov/gene/8114219

http://www.uniprot.org/uniprot/P77608

http://www.ncbi.nlm.nih.gov/pubmed/19786035/