Plasmid

Part:BBa_K1391115:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-30)

pEXPR TRE:Cofilin-TEVp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4549
    Illegal EcoRI site found at 5905
    Illegal EcoRI site found at 5946
    Illegal XbaI site found at 4257
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4835
    Illegal PstI site found at 6483
    Illegal PstI site found at 7132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4549
    Illegal EcoRI site found at 5905
    Illegal EcoRI site found at 5946
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4835
    Illegal PstI site found at 6483
    Illegal PstI site found at 7132
    Illegal NotI site found at 6788
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4549
    Illegal EcoRI site found at 5905
    Illegal EcoRI site found at 5946
    Illegal BglII site found at 6026
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 4583
    Illegal BamHI site found at 6983
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 6768
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4549
    Illegal EcoRI site found at 5905
    Illegal EcoRI site found at 5946
    Illegal XbaI site found at 4257
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4835
    Illegal PstI site found at 6483
    Illegal PstI site found at 7132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4549
    Illegal EcoRI site found at 5905
    Illegal EcoRI site found at 5946
    Illegal XbaI site found at 4257
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 4835
    Illegal PstI site found at 6483
    Illegal PstI site found at 7132
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal AgeI site found at 3456
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1121
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 7215
    Illegal SapI.rc site found at 5719


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

Human, Tobacco etch virus

References