Protein_Domain

Part:BBa_K1390005:Design

Designed by: Stefan Dübel   Group: iGEM14_Braunschweig   (2014-07-03)


mmoY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256


Design Notes

the brick is constructed in the RCF10 standard


Source

Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site

References

Other parts for the complete mmo enzyme:
mmoB: BBa_K1390001
mmoC: BBa_K1390002
mmoD: BBa_K1390003
mmoX: BBa_K1390004
mmoZ: BBa_K1390006