Composite

Part:BBa_K1387006:Design

Designed by: Tri Ekawati Heryanto, Joko Pebrianto Trinugroho, Pande Putu Erawijantari, Nimas Ghasani, Risma Wiharyanti, Gilang Tirta Widi, Oktira Roka Aji   Group: iGEM14_ITB_Indonesia   (2014-09-29)

J23106 - tetO - B0034 - lpp ompA-LC-Cutinase - B1002


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 610
    Illegal AgeI site found at 1222
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion strategy of lpp-ompA on LC Cutinase gene is one of our way to create a whole cell biocatalyst


Source

This part, we desgned through synthetic gene (G-Blocks)

References