Regulatory

Part:BBa_K1387002:Design

Designed by: Tri Ekawati H, Joko Pebrianto T, Pande Putu Erawijantari, Nimas Ghasani, Gilang Tirta Widi   Group: iGEM14_ITB_Indonesia   (2014-09-29)


TetR - B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tetR is tetracycline repressor that bind to operator region and repressing the expression of downstream gene. In our system, the downstream gene is a fusion of lpp’-ompA-LC Cutinase. We combine tetR with double terminator B0015 as our self regulatory module.

Source

We got this part from kit plate iGEM 2014

References