Part:BBa_K1381017:Experience

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Applications of BBa_K1381017

The Killing system
We, the Uppsala iGEM Team 2014, designed the killing system to secret the colicin Fy with the help of a secretion tag, the USP45 BBa_K1381018. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice to it. This part contains the restriction sites NgoMIV at the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site at the start of the sequence it is possible to fuse other sequences if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site. Read more about the system in our [http://2014.igem.org/Team:Uppsala/Project_Killing wiki].

User Reviews

UNIQ067ad0f3de243ed8-partinfo-00000000-QINU UNIQ067ad0f3de243ed8-partinfo-00000001-QINU