Plasmid

Part:BBa_K138000:Design

Designed by: Amrit Misra   Group: iGEM08_Michigan   (2008-10-09)

NifHp in BioBrick Compatible Leucine Landing Pad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Landing pad has ampicillin and chloramphenicol resistance; see protocol on Michigan's wiki on how to get the things in the landing pad on the chromosome. To subclone genes behind the promoter, there are a few options. Cut this part with NdeI/SpeI, NdeI/PstI, or Spe/PstI.

Source

The Leucine Landing Pad was built by Dong Eun Chang, a former member of the Ninfa Lab. nifHp was a PCR product, with the template being pRT22, a nifH-lacZ recorder plasmid. This construct was provided to us by Dr. Ray Dixon of the John Innes Center in the United Kingdom.

References