Part:BBa_K1378023:Design

TorA-MlrA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 376
  Illegal AgeI site found at 505
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is used to test the secretion efficiency of MlrA by different signal peptide and thus choose a best way of secretion.

Source

MlrA coding sequence is obtained by DNA synthesis and is fused with TorA signal peptide by PCR.