Device
Part:BBa_K1373004:Design
Designed by: Wenxuan Chen Group: iGEM14_SCAU-China (2014-09-23)
nadE and oprF with strong promoter and strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1471
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
cloing via omega-PCR
Source
the promoter is from iGEM distribution kit and the nadE gene is from genome of E.coli MG1655 and oprF gene is from PAO1