Device

Part:BBa_K1373002:Design

Designed by: Wenxuan Chen   Group: iGEM14_SCAU-China   (2014-09-23)


nadE with medium promter and strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

substitute BBa_E0034 in BBa_K608010 with BBa_K1373000 via omega-PCR


Source

the promoter is from iGEM distribution kit and the nadE gene is from genome of E.coli MG1655

References