Intermediate

Part:BBa_K1371032:Design

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-07)

KS-AT1+DH-ER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2997
    Illegal BamHI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 685
    Illegal NgoMIV site found at 718
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 1900
    Illegal NgoMIV site found at 2842
    Illegal NgoMIV site found at 3397
    Illegal AgeI site found at 3
    Illegal AgeI site found at 349
    Illegal AgeI site found at 928
    Illegal AgeI site found at 3571
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2185
    Illegal SapI.rc site found at 1020


Design Notes

Source

Saccharopolyspora erythraea

References

[1]Cane, David E. Programming of erythromycin biosynthesis by a modular polyketide synthase [J]. Journal of Biological Chemistry, 2010, 285.36: 27517-27523.

[2]Komaki, Hisayuki, et al. Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species [J]. BMC genomics 15.1 (2014): 323.

[3]Pfeifer, Blaine A., et al. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli [J]. Science, 2001, 291.5509: 1790-1792.

[4]Tae, Hongseok, Jae Kyung Sohng, and Kiejung Park. Development of an analysis program of type I polyketide synthase gene clusters using homology search and profile hidden Markov model [J]. Journal of microbiology and biotechnology, 2009, 19.2: 140-146.

[5]Cortes, Jesus, et al. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea [J]. 1990: 176-178.

[6]Khosla, Chaitan, Shiven Kapur, and David E. Cane. Revisiting the modularity of modular polyketide synthases [J]. Current opinion in chemical biology, 2009, 13.2: 135-143.

[7]Menzella, Hugo G., et al. Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster [J]. Journal of Industrial Microbiology and Biotechnology,2006, 33.1: 22-28.

[8]Oliynyk, Markiyan, et al. A hybrid modular polyketide synthase obtained by domain swapping [J]. Chemistry & biology,1996, 3.10: 833-839.

[9]Lau, Janice, David E. Cane, and Chaitan Khosla. Substrate specificity of the loading didomain of the erythromycin polyketide synthase [J]. Biochemistry, 2000, 39.34: 10514-10520.

[10]Nowak-Thompson, Brian, et al. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5 [J]. Journal of bacteriology, 1999, 181.7: 2166-2174.

[11]Caffrey, Patrick, et al. Amphotericin biosynthesis in Streptomyces nodosus deductions from analysis of polyketide synthase and late genes [J]. Chemistry & biology,2001, 8.7: 713-723. [12]Dunn, Briana J., et al. Comparative analysis of the substrate specificity of trans-versus cis-acyltransferases of assembly line polyketide synthases [J]. Biochemistry,2014.

[13]Jiang, Ming, and Blaine A. Pfeifer. Metabolic and pathway engineering to influence native and altered erythromycin production through E. Coli [J]. Metabolic engineering, 2013, 19: 42-49. [14]Chen, Xianzhong, et al. Metabolic engineering of Escherichia coli: A sustainable industrial platform for bio-based chemical production [J]. Biotechnology advances, 2013, 31.8: 1200-1223.