Composite

Part:BBa_K1371000:Design

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-07)

DEBS1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1878
    Illegal BamHI site found at 6423
    Illegal BamHI site found at 9273
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 558
    Illegal NgoMIV site found at 2041
    Illegal NgoMIV site found at 2413
    Illegal NgoMIV site found at 2446
    Illegal NgoMIV site found at 2919
    Illegal NgoMIV site found at 3628
    Illegal NgoMIV site found at 4501
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1731
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2656
    Illegal AgeI site found at 4377
    Illegal AgeI site found at 4447
    Illegal AgeI site found at 5074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1483
    Illegal BsaI.rc site found at 3913
    Illegal SapI.rc site found at 2748


Design Notes

Candidate selection

Biosynthesis of polyketides like erythromycin, tacrolimus and many others are guided by modular PKS genes. These genes encode large enzymes consisting of modules of domains, forming an "assembly line" to extend the ketide units to form polyketides. Among the known PKS genes, the genes coding for erythromycin producing polyketide synthethase have been extensively and deeply studied, and there are numerous information sources. Therefore, we chose a truncated erythromycin-producing polyketide synthase which has been proved to produce a simple triketide lactone 1B.[2]


Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.