Part:BBa_K137076:Experience

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Applications of BBa_K137076

• Strain: This part will only work in a catalase deficient strain of E. coli. Our testing strain, JI377, is DE(katE, katG, ahp). No H₂O₂ is produced in a catalase proficient strain, such as DH10B.
• Culture conditions: Saturated LB culture backdiluted 1:100 into SOC +1mM IPTG. Let shake at 37C until the culture reaches an OD600 = 0.8. Bring culture to 10nM AHL and let shake at 37 C for 3 hrs. Pellet cells and assay supernatant for hydrogen peroxide.
• Results: Under these conditions the supernatant had ~50 uM H₂O₂ prior to induction and ~200 uM ~200uM H₂O₂ 3hrs post induction with AHL.

User Reviews

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