Reporter
Part:BBa_K137020:Design
Designed by: Allen Lin Group: iGEM08_Caltech (2008-07-21)
GFP with (AC)21 repeat after start codon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684
Design Notes
With 21 AC repeats, the GFP sequence is in frame with respect to the start codon, so a functional GFP should be transcribed. The number of AC repeats was limited by maximum length of synthesized primers.
Source
The dinucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.