Deleted
Sample Discontinued
Experience: None
Not Used
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Coding

Part:BBa_K137002:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-06-20)


L.lactis folate gene cluster


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 4093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 4093
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 4093
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 4093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 4093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2465


Design Notes

We aren't certain whether or not the L.lactis RBS will work in E.coli.


Source

L.lactis genome

References