Plasmid

Part:BBa_K1369009:Design

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pBAD LamA, pLamA-GFP, RR with no recruitment


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 6967
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3656
    Illegal NheI site found at 4906
    Illegal NheI site found at 5454
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8156
    Illegal NotI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3656
    Illegal BglII site found at 4929
    Illegal XhoI site found at 5679
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 6967
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3656
    Illegal XbaI site found at 6967
    Illegal SpeI site found at 3662
    Illegal PstI site found at 8156
    Illegal NgoMIV site found at 2275
    Illegal NgoMIV site found at 5432
    Illegal NgoMIV site found at 7422
    Illegal NgoMIV site found at 7705
    Illegal AgeI site found at 2435
    Illegal AgeI site found at 4690
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6682
    Illegal BsaI site found at 6860
    Illegal BsaI site found at 8793
    Illegal BsaI.rc site found at 6518
    Illegal SapI site found at 4672
    Illegal SapI site found at 7645
    Illegal SapI site found at 7855


Design Notes

There is no scaffold protein in this plasmid, so it will give a negative result when testing the two component system without a ligand.


Source

Lactobacillus plantarum WCFS1

References