Plasmid

Part:BBa_K1369008:Design

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pCon FsrC, constituatively expressed non-inhibited HK with 4 SH3 domains


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 2693
    Illegal XbaI site found at 4719
    Illegal SpeI site found at 7
    Illegal PstI site found at 2699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 42
    Illegal NheI site found at 802
    Illegal SpeI site found at 7
    Illegal PstI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 80
    Illegal BamHI site found at 2300
    Illegal XhoI site found at 2309
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 2693
    Illegal XbaI site found at 4719
    Illegal SpeI site found at 7
    Illegal PstI site found at 2699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 2693
    Illegal XbaI site found at 4719
    Illegal SpeI site found at 7
    Illegal PstI site found at 2699
    Illegal AgeI site found at 3963
    Illegal AgeI site found at 4287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2408
    Illegal BsaI site found at 2586
    Illegal SapI.rc site found at 797
    Illegal SapI.rc site found at 1595
    Illegal SapI.rc site found at 1805
    Illegal SapI.rc site found at 2015
    Illegal SapI.rc site found at 2225


Design Notes

It contains 4 SH3 domains


Source

Enterococcus faecalis

References