Regulatory

Part:BBa_K1369001:Design

Designed by:   Group: iGEM14_Caltech   (2014-08-18)

Repressor-Repressor promoter (LacI and TetR operator sites)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test the logic of this RR promoter, we assembled constructs containing the promoter, a bicistronic RBS (part BBa_J119024), and GFP, in that order, downstream of the promoter.


Source

This part corresponds to the B83 promoter construct used in the paper by Robert Sidney Cox III et al. [1]

References

1. Cox III RS, Surette MG, Elowitz MB (2007) Programming gene expression with combinatorial promoters. Molecular Systems Biology 3: 145