Composite

Part:BBa_K1368003:Design

Designed by: liu Tian   Group: iGEM14_HZAU-China   (2014-09-29)


ptetR+lox66+lacI+plac(R0011)+c1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

c1 protein may be noxious to the E.coli.We had tried many times to ligate the plac and c1 without success.So, we ligated the plac under the part of ptetR+lox66+lacI. With the lacI protein repressed, we made it.


Source

Thanks to HUST-China team and they help us build this part by basic parts from iGEM Registry.

References