Composite
C4

Part:BBa_K1366103:Design

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)


GFP expression under a hTERT promoter (C4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1572


Design Notes

The structure goes like this hTERT // RBS // GFP // Terminador


Source

We obtain the sequence of the hTERT promoter from the part BBa_K404106 and we used the GFP sequence that is available in uniprot and optimized it for mammalian cell expression

References