Translational_Unit

Part:BBa_K1362992:Design

Designed by: Jakob Kreft   Group: iGEM14_Heidelberg   (2014-10-05)


-- No description --


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was designed to be used with to RFC[???]. Parts were amplified from source plasmids with matching overhangs and assembled via CPEC.


Source

The RBS comes from BBa_K1362090. Npu DnaE C-Intein was cloned from pVS41 obtained from Prof. Henning D. Mootz, University of Muenster. BBa_J04450 was used as mRFP selection marker.

References