Protein_Domain
SortA clv.

Part:BBa_K1362454:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

Sortase A recognition/cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Staphylococcus aureus

  1. Proft, Thomas (2010). "Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation." Biotechnology Letters 32 (1): 1–10
  2. Popp, Maximilian W.-L. (2011). "Making and Breaking Peptide Bonds: Protein Engineering Using Sortase." Angewandte Chemie International Edition 50 (22): 5024–32