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Part:BBa_K1362428:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

FLAG tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

???


Source

The amino acid sequence DYKDDDDK [1] was backtranslated and obtained by DNA synthesis.


References

[1] Hopp, Thomas P.; Prickett, Kathryn S.; Price, Virginia L.; Libby, Randell T.; March, Carl J.; Pat Cerretti, Douglas; Urdal, David L.; Conlon, Paul J. (1988). "A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification". Bio/Technology 6 (10): 1204–10