Protein_Domain
SspGyrB(N)

Part:BBa_K1362406:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-06)


SspGyrB N-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.


Source

Part was made using by DNA synthesis. The Sequence is obtained from Liu et al.[1]

References

[1] Lin Y, Li M, Song H, Xu L, Meng Q, et al. (2013) Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins. PLoS ONE 8(4): e59516. doi:10.1371/journal.pone.0059516 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059516