Protein_Domain
NpuDnaE(N)

Part:BBa_K1362400:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-06)


NpuDnaE N-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.

Source

Obtained by PCR amplification from pVS41 by Prof. Henning D. Mootz, University of Muenster.[1]

References

[1] Joachim Zettler, Vivien Schütz, Henning D. Mootz, The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction, FEBS Letters, Volume 583, Issue 5, 4 March 2009, Pages 909-914, ISSN 0014-5793, http://dx.doi.org/10.1016/j.febslet.2009.02.003.

[2] Cheriyan, M., Pedamallu, C. S., Tori, K. & Perler, F. Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues. J. Biol. Chem. 288, 6202–11 (2013).