Tag
MTS

Part:BBa_K1362255:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

MinD MTS tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

C-terminal tag
Note: There are no stop codons included in this sequence.

Source

origin: Bacillus subtilis
The part sequence as described by Szeto et al. [1] was ordered as DNA oligos.


References

[1] Tim H. Szeto et al. Membrane localization of MinD is mediated by a C-terminal motif that is conserved acrosseubacteria, archaea, and chloroplasts PNAS 2002 99 (24) 15693-15698 Published online: 22 May 2005 | doi:10.1073/pnas.232590599