Composite
CircSortA

Part:BBa_K1362002:Design

Designed by: Silvan Schmitz   Group: iGEM14_Heidelberg   (2014-10-09)

Sortase A circularization construct (with His6)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 855
    Illegal AgeI site found at 967
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1144
    Illegal BsaI.rc site found at 70


Design Notes

The mRFP selection marker BBa_J04450, which can be removed by restriction with BsaI, is flanked by TEV protease cleavage site (left) and a sorting signal (right) with a His6 tag. By Golden gate assembly, the selection marker can be replaced by the protein to be circularized.


Source

See sources of subparts.


References