Protein_Domain

Part:BBa_K1361999:Design

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-03)


Curli Fiber major monomer-CsgA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.


Source

PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.

References